Ana J. Pererz- Berna, Nuria Bensey-Cases, María José Rodríguez, Ricardo Valcarcel, José L. Carrascosa, Pablo Gastaminza, Eva Pereiro
Monitoring reversion of hepatitis C virus-induced cellular alterations by direct-acting antivirals using cryo soft X-ray tomography and infrared microscopy, Acta Cryst. D77, 1365-1377, 2021

Hepatitis C virus (HCV) is an enveloped RNA virus. One of the hallmarks of HCV infection is a rearrangement of the host cell membranes, known as the ‘membranous web’.

The research paper below, published by partners in the CoCID consortium, describes how Cryo-SXT was used to help investigate the morphology of the membranous rearrangements induced in HCV replicon-harbouring cells in conditions close to the living physiological state. All morphological alterations could be reverted by a combination of sofosbuvir/daclatasvir, which are clinically approved antivirals (direct-acting antivirals; DAAs) for HCV infection.

Cryo-SXT helped provide critical information on the chemical nature of specific infection-related structures, which allows specific patterns of the infection process or the DAA-mediated healing process to be distinguished.

Read the full paper here

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